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recombinant human bag3 proteins  (ProSpec)


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    Structured Review

    ProSpec recombinant human bag3 proteins
    Recombinant Human Bag3 Proteins, supplied by ProSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+bag3+proteins/recombinant+human+bag3+proteins/pmc03772389-451-3-6
    Average 90 stars, based on 1 article reviews
    recombinant human bag3 proteins - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    In Vitro:

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes
    Article Snippet: 10 μl of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 μl lysates, followed by incubation at 37°C for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30°C for 1 h in 100 μl reactions containing 1 μg of BAG3, 20 μl of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl 2 and 2 mM ATP. .. The mixtures were then added to 1 μg of recombinant 14-3-3γ proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes.
    Article Snippet: 10 ml of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 ml lysates, followed by incubation at 37 C̊ for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30 C̊ for 1 h in 100 ml reactions containing 1 mg of BAG3, 20 ml of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl2 and 2 mM ATP. .. The mixtures were then added to 1 mg of recombinant 14-3-3c proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Phospho-proteomics:

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes
    Article Snippet: 10 μl of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 μl lysates, followed by incubation at 37°C for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30°C for 1 h in 100 μl reactions containing 1 μg of BAG3, 20 μl of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl 2 and 2 mM ATP. .. The mixtures were then added to 1 μg of recombinant 14-3-3γ proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes.
    Article Snippet: 10 ml of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 ml lysates, followed by incubation at 37 C̊ for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30 C̊ for 1 h in 100 ml reactions containing 1 mg of BAG3, 20 ml of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl2 and 2 mM ATP. .. The mixtures were then added to 1 mg of recombinant 14-3-3c proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes
    Article Snippet: 10 μl of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 μl lysates, followed by incubation at 37°C for 30 min. .. Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30°C for 1 h in 100 μl reactions containing 1 μg of BAG3, 20 μl of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl 2 and 2 mM ATP. .. The mixtures were then added to 1 μg of recombinant 14-3-3γ proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Binding Assay:

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes
    Article Snippet: 10 μl of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 μl lysates, followed by incubation at 37°C for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30°C for 1 h in 100 μl reactions containing 1 μg of BAG3, 20 μl of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl 2 and 2 mM ATP. .. The mixtures were then added to 1 μg of recombinant 14-3-3γ proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes.
    Article Snippet: 10 ml of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 ml lysates, followed by incubation at 37 C̊ for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30 C̊ for 1 h in 100 ml reactions containing 1 mg of BAG3, 20 ml of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl2 and 2 mM ATP. .. The mixtures were then added to 1 mg of recombinant 14-3-3c proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Recombinant:

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes
    Article Snippet: 10 μl of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 μl lysates, followed by incubation at 37°C for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30°C for 1 h in 100 μl reactions containing 1 μg of BAG3, 20 μl of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl 2 and 2 mM ATP. .. The mixtures were then added to 1 μg of recombinant 14-3-3γ proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes.
    Article Snippet: 10 ml of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 ml lysates, followed by incubation at 37 C̊ for 30 min. .. In vitro phosphorylation and binding of BAG3 proteins Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30 C̊ for 1 h in 100 ml reactions containing 1 mg of BAG3, 20 ml of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl2 and 2 mM ATP. .. The mixtures were then added to 1 mg of recombinant 14-3-3c proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.

    Article Title: 14-3-3 protein targets misfolded chaperone-associated proteins to aggresomes
    Article Snippet: 10 μl of calf intestinal alkaline phosphatase (New England BioLabs) was added to 500 μl lysates, followed by incubation at 37°C for 30 min. .. Phosphorylation of recombinant human BAG3 proteins (ProSpec) was carried out at 30°C for 1 h in 100 μl reactions containing 1 μg of BAG3, 20 μl of whole-cell extract from tsA201 cells, 20 mM Tris-HCL (pH, 7.5), 120 mM NaCl, 25 mM NaF, 25 mM KCl, 10 mM EDTA, 10 mM MgCl 2 and 2 mM ATP. .. The mixtures were then added to 1 μg of recombinant 14-3-3γ proteins (ProSpec), and 14-3-3 immunoprecipitation and western blot analysis were carried out as described above.



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    Image Search Results


    Schematic illustration of the BAG3 protein sequence. Gray boxes highlight specific domains: WW (with two conserved tryptophan residues), IPV (a tripeptide motif isoleucine‐proline‐valine), PxxP (a proline‐rich motif). Black bars indicate LiP (limited proteolysis) peptides identified as involved in binding interactions, located from amino acids 231 to 249, 432 to 445, and 461 to 473, respectively.

    Journal: Chemmedchem

    Article Title: Identification of a New Interesting BAG3 Modulator Able to Disrupt Cancer‐Related Pathways

    doi: 10.1002/cmdc.202500310

    Figure Lengend Snippet: Schematic illustration of the BAG3 protein sequence. Gray boxes highlight specific domains: WW (with two conserved tryptophan residues), IPV (a tripeptide motif isoleucine‐proline‐valine), PxxP (a proline‐rich motif). Black bars indicate LiP (limited proteolysis) peptides identified as involved in binding interactions, located from amino acids 231 to 249, 432 to 445, and 461 to 473, respectively.

    Article Snippet: Recombinant human BAG3 protein (Bcl2‐associated athanogene 3) was obtained from Novus Biologicals (Littleton, Colorado), while the BAG3 domain (BAG3‐BD) was sourced from ARETA International S.r.l. (Gerenzano, Italy).

    Techniques: Sequencing, Binding Assay

    Binding mode of a) SP18 (colored by atom type: C green, N blue, O red), b) compound 1 (colored by atom type: C faded magenta, N blue, O red), c) compound 2 (colored by atom type: C orange, N blue, O red), d) compound 5 (colored by atom type: C azure, N blue, O red), e) compound 6 (colored by atom type: C violet, N blue, O red, Cl green), f) compound 7 (colored by atom type: C yellow, N blue, O red, Br dark red), g) compound 8 (colored by atom type: C cyan, N blue, O red, I magenta). H bonds, halogen bonds, and π interactions are reported in yellow, violet, and green dotted lines, respectively. Residues corresponding to the sequence of murine BAG3 BD as renumbered in the Protein Data Bank (PDB code: 1UK5) and corresponding residues belonging to human BAG3 BD are reported in black and red, respectively.

    Journal: Chemmedchem

    Article Title: Identification of a New Interesting BAG3 Modulator Able to Disrupt Cancer‐Related Pathways

    doi: 10.1002/cmdc.202500310

    Figure Lengend Snippet: Binding mode of a) SP18 (colored by atom type: C green, N blue, O red), b) compound 1 (colored by atom type: C faded magenta, N blue, O red), c) compound 2 (colored by atom type: C orange, N blue, O red), d) compound 5 (colored by atom type: C azure, N blue, O red), e) compound 6 (colored by atom type: C violet, N blue, O red, Cl green), f) compound 7 (colored by atom type: C yellow, N blue, O red, Br dark red), g) compound 8 (colored by atom type: C cyan, N blue, O red, I magenta). H bonds, halogen bonds, and π interactions are reported in yellow, violet, and green dotted lines, respectively. Residues corresponding to the sequence of murine BAG3 BD as renumbered in the Protein Data Bank (PDB code: 1UK5) and corresponding residues belonging to human BAG3 BD are reported in black and red, respectively.

    Article Snippet: Recombinant human BAG3 protein (Bcl2‐associated athanogene 3) was obtained from Novus Biologicals (Littleton, Colorado), while the BAG3 domain (BAG3‐BD) was sourced from ARETA International S.r.l. (Gerenzano, Italy).

    Techniques: Binding Assay, Sequencing

    Mesenteric vascular Western blot graph ( A ) and analysis of 4-HNE ( B ), CaSR ( C ), p-eNOS ( D ) and BAG3 ( E ) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F ) and vasodilator (acetylcholine, Ach, G ) is displayed in CON and STZ groups. All data are presented as the mean ± SEM ( n = 3) using the single values in each test. * p < 0.05 vs. CON group.

    Journal: Antioxidants

    Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

    doi: 10.3390/antiox9080679

    Figure Lengend Snippet: Mesenteric vascular Western blot graph ( A ) and analysis of 4-HNE ( B ), CaSR ( C ), p-eNOS ( D ) and BAG3 ( E ) are demonstrated. Microvascular reactivity from mesenteric arterioles to a vasoconstrictor (norepinephrine, NE, F ) and vasodilator (acetylcholine, Ach, G ) is displayed in CON and STZ groups. All data are presented as the mean ± SEM ( n = 3) using the single values in each test. * p < 0.05 vs. CON group.

    Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

    Techniques: Western Blot

    Western blot and immunohistochemistry of BAG3 ( A , B ) and Bcl-2 ( C , D ) in IR myocardium. BAG3 and Bcl-2 expression is significantly decreased in the STZ group than those in the CON group. The BAG3 and Bcl-2 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 and Bcl-2 expression than IR CON hearts. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON.

    Journal: Antioxidants

    Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

    doi: 10.3390/antiox9080679

    Figure Lengend Snippet: Western blot and immunohistochemistry of BAG3 ( A , B ) and Bcl-2 ( C , D ) in IR myocardium. BAG3 and Bcl-2 expression is significantly decreased in the STZ group than those in the CON group. The BAG3 and Bcl-2 expression is also significantly reduced in the IR CON and IR STZ groups as compared with the CON group. IR STZ hearts further depress BAG3 and Bcl-2 expression than IR CON hearts. Data are expressed as mean ± SEM ( n = 3) using the single values. * p < 0.05 vs. CON, a p < 0.05 vs. STZ, b p < 0.05 vs. IR CON.

    Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

    Techniques: Western Blot, Immunohistochemistry, Expressing

    The summary diagram is demonstrated. Diabetes enhanced cardiovascular damage by suppression of antioxidant defense mechanisms including Nrf-2/HO-1, BAG3 and Bcl-2 and increased oxidative stress including vascular and cardiac reactive oxygen species (ROS). In response to myocardial IR injury, diabetes further decreased antioxidant defense mechanisms and enhanced oxidative stress/injury, leading to cardiovascular dysfunction and injury.

    Journal: Antioxidants

    Article Title: Diabetes Upregulates Oxidative Stress and Downregulates Cardiac Protection to Exacerbate Myocardial Ischemia/Reperfusion Injury in Rats

    doi: 10.3390/antiox9080679

    Figure Lengend Snippet: The summary diagram is demonstrated. Diabetes enhanced cardiovascular damage by suppression of antioxidant defense mechanisms including Nrf-2/HO-1, BAG3 and Bcl-2 and increased oxidative stress including vascular and cardiac reactive oxygen species (ROS). In response to myocardial IR injury, diabetes further decreased antioxidant defense mechanisms and enhanced oxidative stress/injury, leading to cardiovascular dysfunction and injury.

    Article Snippet: The expression of β-actin (#4970, 1:1000; Cell Signaling Technology, MA, USA), 4-HNE (bs-6313R, 1:1000; Bioss, MA, USA), BAG3 (NBP2-27398, 1:2000; Novus, CO, USA), Bcl-2 (bs-0032R, 1:1000; Bioss, MA, USA), caspase 3 (bs-0081R, 1:1000; Bioss, MA, USA), p-eNOS (ser1177, 1:1000; Cell Signaling Technology, MA, USA) and PARP (#9532, 1:1000; Cell Signaling Technology, MA, USA) from the damaged heart infarct areas after ischemia/reperfusion injury were evaluated by Western blotting as described before [ ].

    Techniques: